Cytochrome c Release Assay Kit from Abcam

Product Specs
ItemCytochrome c Release Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65311
Quantity100 test
Sample TypeTissue, Adherent cells, Suspension cells
Time3h 0m
ApplicationsCellular Assay
Gene Namecycs
ReactivityHuman, Mouse, Rat
TypeDirect
NCBI Gene AliasesCYC, HCS, THC4
TargetCytochrome c

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) ER stress induces caspase‐2‐tBID‐GSDME‐dependent cell death in neurons lytically infected with herpes simplex virus type 2The EMBO JournalOctober 4, 2023Fanghui Ren, Ryo Narita, Ahmad S Rashidi, Stefanie Fruhwürth, Zongliang Gao, Rasmus O Bak, Martin K Thomsen, Georges MGM Verjans, Line S Reinert, Søren R Paludan(Abcam, ab39830), caspase 3 activity assay kit (Abcam, ab39401), cytochrome c release assay kit (Abcam, ab65311), annexin V conjugates for apoptosis detection (Invitrogen™, A13201), and CyQUANT™ LDH cytotoxicity

(2) Esophageal cancer stem cells reduce hypoxia-induced apoptosis by inhibiting the GRP78-perk-eIF2α-ATF4-CHOP pathway in vitroJournal of Gastrointestinal OncologyAugust 31, 2023Ruijiang Lin, Minjie Ma, Biao Han, Ya Zheng, Yuping Wang, Yongning Zhou(CA1020; Solarbio Co.); cycle detection kit (CA1510; Solarbio Co.); Cyt C releasing apoptosis assay kit (Ab65311; Abcam, Cambridge, MA, USA); radioimmunoprecipitation assay (RIPA) lysis buffer (P0013C; Beyotime Co.,

(3) Elephant TP53-RETROGENE 9 induces transcription-independent apoptosis at the mitochondriaCell death discoveryFebruary 16, 2023Preston AJ, Rogers A, Sharp M, Mitchell G, Toruno C, Barney BB, Donovan LN, Bly J, Kennington R, Payne E, et al.constructs. Cells were harvested after 48 h and processed using the Cytochrome C Release assay from Abcam (ab65311). The cytosolic and mitochondrial fractions were quantified by Bradford protein assay, then resuspended

(4) Selective Induction of Intrinsic Apoptosis in Retinoblastoma Cells by Novel Cationic Antimicrobial DodecapeptidesPharmaceuticsNovember 18, 2022Vishnu Suresh Babu, Atish Kizhakeyil, Gagan Dudeja, Shyam S. Chaurasia, Veluchami Amutha Barathi, Stephane Heymans, Navin Kumar Verma, Rajamani Lakshminarayanan, Arkasubhra Ghoshfollowed by mitochondrial extraction buffer using the cytochrome c releasing apoptosis assay kit (cat#ab65311, Abcam, Cambridge, UK) as per the manufacturer’s protocol. 20 µg of mitochondrial and cytosol protein

(5) Label-free chemical imaging of cytochrome P450 activity by Raman microscopyCommunications BiologyAugust 22, 2022Menglu Li, Yasunori Nawa, Seiichi Ishida, Yasunari Kanda, Satoshi Fujita, Katsumasa Fujitaanti-CYP3A4 (1:2000, Sigma-Aldrich), rabbit anti-cyt b5 (1:1000, Abcam), mouse anti-cyt c (1:200, Abcam, ab65311), and rabbit anti-β-actin (1:1000; 4970 S; CST) primary antibody overnight. The following day, horseradish

(6) Lysosomes Dysfunction Causes Mitophagy Impairment in PBMCs of Sporadic ALS PatientsCellsApril 9, 2022Matteo Bordoni et al.(ab56788),mouse,1:500,Abcam MFN1 (ab57602),mouse,1:1000,Abcam OPA1 (ab157457),rabbit,1:1000,Abcam CYT C (ab65311),mouse,1:200,Abcam BCL-2 (ab59348),rabbit,1:200,Abcam Antibodies used in the WB analysis. We reported

(7) Platelet Mitochondria Transplantation Rescues Hypoxia/Reoxygenation-Induced Mitochondrial Dysfunction and Neuronal Cell Death Involving the FUNDC2/PIP3/Akt/FOXO3a AxisCell TransplantationJune 9, 2021Chun Shi, Han Guo, Xintong Liuleakage of cytochrome c (Cyto C) was analyzed by Western blotting with Cyto C-releasing assay kit (ab65311, Abcam, UK). Western blotting was conducted on whole-cell, cytoplasmic and mitochondrial lysates with

(8) γ‑irradiated prednisolone promotes apoptosis of liver cancer cells via activation of intrinsic apoptosis signaling pathwayMolecular Medicine ReportsApril 7, 2021Fatuma Jumapili Ramadhani, Seong Hee Kang, Remigius Ambrose Kawala, Byung Yeoup Chung, Hyoung-Woo Bai, Bo Sun Kang(BCA) reagent was obtained from Thermo Fisher Scientific, Inc. The cytochrome c release kit (cat. no. ab65311) was from Abcam. Anti-GAPDH, anti-cleaved caspase 3, anti-cleaved caspase 9, anti-caspase 9, anti-cleaved

(9) Collapsin response mediator protein 4 enhances the radiosensitivity of colon cancer cells through calcium‑mediated cell signalingOncology reportsApril 1, 2021Park SY, Kim JT, Park ES, Hwang YS, Yoon HR, Baek KE, Jung H, Yoon SR, Kim BY, Cho HJ, et al.flow cytometry. Measurement of cytochrome c release The cytochrome c- releasing apoptosis assay kit (Abcam; ab65311) was used for detecting cytochrome c translocation from mitochondria into the cytosol. After irradiated

(10) Leptin reduces in vitro cementoblast mineralization and survival as well as induces PGE2 release by ERK1/2 commitmentClinical oral investigationsApril 1, 2021Ruiz-Heiland G, Yong JW, von Bremen J, Ruf S.Thermo-Fisher) dilution 1:1000; phospho-STAT3 Ser727 (OPA1-03007, ThermoFisher) dilution 1:500; Cytochrome C (ab65311, Abcam) dilution 1:1000; cPLA2α (orb100010, BIOZOL) dilution 1:1000; cPLA2β (ab198898, Abcam) dilution

(11) Brain immune cells undergo cGAS/STING-dependent apoptosis during herpes simplex virus type 1 infection to limit type I IFN productionJournal of Clinical InvestigationJanuary 4, 2021Line S. Reinert et al.cytosolic fractions of stimulated cells were separated using 14 the Cytochrome C Release Assay Kit (Abcam, ab65311) according to the manufacturer’s protocol, and 30 μg of the cytosolic and mitochondrial fractions was

(12) Amino acid depletion triggered by ʟ-asparaginase sensitizes MM cells to carfilzomib by inducing mitochondria ROS-mediated cell deathBlood AdvancesSeptember 22, 2020Debora Soncini et al.from mitochondria into cytosol during apoptosis was assayed using a Cytochrome c Release Assay Kit (ab65311; Abcam), according to the manufacturer’s instructions. Cell viability and apoptosis assay The viability

(13) Adrenergic-Independent Signaling via CHRNA2 Regulates Beige Fat ActivationDevelopmental CellJuly 1, 2020Heejin Jun et al.Assays,Critical Commercial Assays,Critical Commercial Assays Cytochrome c Release Assay Kit,Abcam,Cat# ab65311 Citrate Synthase Activity Assay Kit,Cayman chemical,Cat# 701040 L-Lactate Assay Kit,Cayman chemical,Cat#

(14) The TLK1/Nek1 axis contributes to mitochondrial integrity and apoptosis prevention via phosphorylation of VDAC1Cell cycle (Georgetown, Tex.)February 1, 2020Singh V, Khalil MI, De Benedetti A.Chen, UCI), Cyclin A (Catalog No. sc-271645), Cyclin B (Catalog No. sc-245), Cytochrome C (Catalog No. ab65311) Abcam, Complex COX IV (Catalog No. sc-376731), actin (Catalog No. sc-8432), GAPDH (Catalog No. 2118S)

(15) 3-Bromopyruvate alleviates the development of monocrotaline-induced rat pulmonary arterial hypertension by decreasing aerobic glycolysis, inducing apoptosis, and suppressing inflammationChinese Medical JournalJanuary 5, 2020Jie Liu, Wang Wang, Lei Wang, Xian-Mei Qi, Yu-Hui Sha, Ting Yangapoptosis in tissues, cell apoptosis was determined using a Cyto C release apoptosis assay kit (Catalog No. ab65311; Abcam) according to the manufacturer's instructions. Statistical analysis Data are expressed as the

(16) Induction of ROS‑mediated cell death and activation of the JNK pathway by a sulfonamide derivativeInternational journal of molecular medicineOctober 1, 2019Ahmad R, Vaali-Mohammed MA, Elwatidy M, Al-Obeed O, Al-Khayal K, Eldehna WM, Abdel-Aziz HA, Alafeefy A, Abdulla M.were exposed overnight to the following primary antibodies at 4˚C: Anti‑cytochrome c (1:200; Abcam, ab65311), anti‑p53 (Santa Cruz Biotechnology Inc., sc‑99), anti‑Bcl‑2‑associated X protein (Bax; Santa Cruz Biotechnology Inc

(17) Synthetic Essentiality of Metabolic Regulator PDHK1 in PTEN-Deficient Cells and CancersCell reportsAugust 27, 2019Chatterjee N, Pazarentzos E, Mayekar MK, Gui P, Allegakoen DV, Hrustanovic G, Olivas V, Lin L, Verschueren E, Johnson JR, et al.Assay,Promega,Cat# E2610 Malachite Green Assay,Echelon,Cat# K1500 Cytochrome c Release Assay,Abcam,Cat# ab65311 Deposited Data,, Raw and analyzed microarray data,This paper,GEO: GSE121217 Raw and analyzed phospho-proteomics

(18) Mesenchymal stromal cell-derived exosomes improve mitochondrial health in pulmonary arterial hypertensionAmerican Journal of Physiology-Lung Cellular and Molecular PhysiologyMay 1, 2019Sarah E. Hogan, Maria Pia Rodriguez Salazar, John Cheadle, Rachel Glenn, Carolina Medrano, Thomas H. Petersen, Roger M. Ilaganthe cytochrome-c releasing apoptosis assay according to manufacturer instructions (Abcam, cat. no. ab65311). Tissue was processed on ice using kit-provided mitochondrial lysis buffers and a Tissue-Tearor 985370

(19) G418 induces programmed cell death in Acanthamoeba through the elevation of intracellular calcium and cytochrome c translocationParasitology ResearchFebruary 1, 2019Zisis Koutsogiannis, Ewan T. MacLeod, Sutherland K. Macivermitochondria Cytochrome c release was assayed using a commercial kit (Abcam’s cytochrome c releasing apoptosis assay kit (ab65311)). Acanthamoeba trophozoites were incubated with IC90 G418 in NSB at 37 °C for predetermined period

(20) Kenalog modified by ionizing radiation induces intrinsic apoptosis mediated by elevated levels of reactive oxygen species in melanoma cancerOncology ReportsDecember 19, 2018Remigius Ambrose Kawala, Fatuma Jumapili Ramadhani, Hyo Jin Choi, Eun‑Hee Lee, Chul‑Hong Park, Byung Yeoup Chung, Hyoung‑Woo Baicytosolic and mitochondria fractions were separated by Cytochrome c Release Apoptosis Assay kit (cat. no. ab65311; Abcam, Cambridge, UK) following the manufacturer's instructions. Cell debris was removed by centrifugation

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Cytochrome c Release Assay Kit from Abcam

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Cytochrome c Release Assay Kit from Abcam

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